Abstract

Background and Objective: Burn wound is a serious issue in health because of the outcome results the physical disability, influencing the morbidity of the patient, high cost of burn wound treatment. Treatment of full depth burn has been done in various ways by injecting growth factors and skin grafts, but the result has not provided satisfactory, it’s due to the formation of scar tissue, the absence of hair follicles and sebaceous tissue. Bone marrow stem cells (BM-MSCs) are strong potential cell in skin tissue regeneration. Study on BM-MSCs administration to burn wound is unclear and this study was performed to identify the formation of granulation tissue and to investigate the expression level of transforming growth factor beta 3 (TGF-β3) and matrix metalloproteinase-9 expression (MM-9). The TGF-β3 plays a role in reducing scar tissue formation and MMP9 serves as collagen destruction. Materials and Methods: Ten Wistar rats were divided into two groups, i,e., control group was treated with PBS and treated group was injected with BM-MSCs. A burn wound was made on dorsal area of rat. On day 14 after treatment, burn wound was investigated; immunohistochemical examination was performed to identify granulation tissue formation, TGF-β3 and MMP-9 expression. Results: The administration of BM-MSCs on day 14 accelerated the burns healing in rat and increased the expression level of TGF-β3 (7.32) and MMP-9 (6.26) significantly, compared with in control group (2.64 and 3.98). Conclusion: The BM-MSCs accelerated the burns healing in rat by elevating the expression level of TGF-β3 and MMP-9 which acts in proliferation phase in wound healing.