Abstract
Background and Objective: Amiodarone-induced kidney damage is associated with an increase in oxidant and proinflammatory cytokines. This study was planned to investigate the effect of thiamine pyrophosphate (TPP) on amiodarone-induced oxidative and inflammatory kidney damage in rats on both a biochemical and histopathological basis. Materials and Methods: Albino Wistar-type male rats (6 rats per group) were divided into healthy (HG), amiodarone (ADG) and amiodarone+TPP (TAG) groups. The TPP (20 mg kg–1) and amiodarone (50 mg kg–1) treatment continued for 14 days. Analyzes of oxidant, antioxidant and proinflammatory cytokines in excised kidney tissues and creatinine and blood urea nitrogen in serum were performed. Results: The amiodarone treated rats showed an increase in malondialdehyde, tumor necrosis factor, interleukin 1 beta, interleukin 6 levels, serum creatinine and blood urea nitrogen in the kidney tissue while a decrease was observed in total glutathione as compared to healthy animals (p<0.001). In addition, histopathological damage occurred in the kidney tissues of the ADG group. It was determined that TPP prevented biochemical changes and histopathological damage significantly (p<0.001). Conclusion: By acting as an antioxidant and anti-inflammatory, TPP might protect kidney tissue from amiodarone-induced damage.